Keratin and S100 calcium-binding proteins are major constituents of the bovine teat canal lining

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Keratin and S100 calcium-binding proteins are major constituents of the bovine teat canal lining

The bovine teat canal provides the first-line of defence against pathogenic bacteria infecting the mammary gland, yet the protein composition and host-defence functionality of the teat canal lining (TCL) are not well characterised. In this study, TCL collected from six healthy lactating dairy cows was subjected to two-dimensional electrophoresis (2-DE) and mass spectrometry. The abundance and l...

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Effect of machine milking on bovine teat sinus injury and teat canal keratin

Introduction In Ireland, cows are generally milked with milking machines with 3.2kg clusters, with wide-bore tapered liners (31.6mm – 20.6mm) and 4x0 pulsation (WB). Outside of Ireland, most milking machines have 1.65kg clusters, narrow-bore liners (25.0mm – 20.0mm) and 2x2 pulsation (NB). O’Callaghan (2001) showed that the mouthpiece cavity vacuum was higher with wide-bore tapered liners than ...

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S100 Calcium Binding Proteins and Ion Channels

S100 Ca(2+)-binding proteins have been associated with a multitude of intracellular Ca(2+)-dependent functions including regulation of the cell cycle, cell differentiation, cell motility and apoptosis, modulation of membrane-cytoskeletal interactions, transduction of intracellular Ca(2+) signals, and in mediating learning and memory. S100 proteins are fine tuned to read the intracellular free C...

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Calcium-binding S100 protein expression in pterygium

PURPOSE Pterygium is an ocular surface disease of unknown etiology associated with epithelial and fibrovascular outgrowth from the conjunctiva onto the cornea. S100 proteins are calcium-activated signaling proteins that interact with other proteins to modulate biological functions such as cell migration, proliferation, and differentiation. The aim of this study was to investigate the presence o...

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Calcium regulation of Ndr protein kinase mediated by S100 calcium-binding proteins.

Ndr is a nuclear serine/threonine protein kinase that belongs to a subfamily of kinases identified as being critical for the regulation of cell division and cell morphology. The regulatory mechanisms that control Ndr activity have not been characterized previously. In this paper, we present evidence that Ndr is regulated by EF-hand calcium-binding proteins of the S100 family, in response to cha...

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ژورنال

عنوان ژورنال: Veterinary Research

سال: 2015

ISSN: 1297-9716

DOI: 10.1186/s13567-015-0227-7